This study investigated GSE for antibacterial activity at varying time intervals and concentration levels and tissue toxicity at varying concentrations in an effort to determine if a concentration existed that was both microbicidal and nontoxic and in what period of time. N2 - Objectives: Recent testimonials report grapefruit-seed extract, or GSE® (Citricidal®) to be effective against more than 800 bacterial and viral strains, 100 strains of fungus, and a large number of single and multicelled parasites. Mechanism of action and in vitro toxicityĬopyright 2018 Elsevier B.V., All rights reserved. T1 - The effectiveness of processed grapefruit-seed extract as an antibacterial agent It was evident that GSE disrupts the bacterial membrane and liberates the cytoplasmic contents within 15 minutes after contact even at more dilute concentrations.", With the aid of scanning transmission electron microscopy (STEM), the mechanism of GSE's antibacterial activity was revealed. Conclusions: The initial data shows GSE to have antimicrobial properties against a wide range of gram-negative and gram-positive organisms at dilutions found to be safe. However, test results indicated that at the 1:512 dilution, GSE remained bactericidal, but completely nontoxic. Results: These tests indicated that from the 1:1 through the 1:128 concentrations, GSE remained toxic as well as bactericidal. In vitro assays with human skin fibroblast cells were also performed at the same dilutions to determine toxicity. Design: Gram-negative and gram-positive isolates were introduced into graduated dilutions of GSE (twofold concentrations ranging from 1:1, through 1:512) for determination of bacterial activity. It was evident that GSE disrupts the bacterial membrane and liberates the cytoplasmic contents within 15 minutes after contact even at more dilute concentrations.Ībstract = "Objectives: Recent testimonials report grapefruit-seed extract, or GSE) to be effective against more than 800 bacterial and viral strains, 100 strains of fungus, and a large number of single and multicelled parasites. Objectives: Recent testimonials report grapefruit-seed extract, or GSE® (Citricidal®) to be effective against more than 800 bacterial and viral strains, 100 strains of fungus, and a large number of single and multicelled parasites.
0 Comments
Leave a Reply. |